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Cryopreservation of cultured plant cells and meristems by vitrification.

[In Japanese. / En japonais.]

Author(s) : SAKAI A.

Summary

The encapsulation/dehydration technique is easy to handle and alleviates the dehydration process. However, the problems are lower rate of survival, later recovery growth and longer dehydration process compared with the vitrification procedure. To solve these problems, a novel cryogenic procedure, encapsulation/vitrification method is presented. In this method, meristems are trapped into alginate-coated beads containing a mixture of 0.4 mole sucrose and 2 moles glycerol and dehydrated with PVS2 for about 100 minutes at 0 deg C. This procedure is easy to handle and produces high survival levels. Thus the method is promising for cryopreserving meristems, hairy roots and somatic embryos.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1998-3666
  • Languages: Japanese
  • Source: Seminar "Adaptation of plants to low temperatures: science and practice".
  • Publication date: 1996
  • Available in the IIR library

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