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A preliminary method for ultra-rapid freezing of the Nicotiana tabacum BY-2 cell line by encapsulation/vitrification.

Number: pap. n. 094

Author(s) : SCHUMACHER H. M., BITTER E., HEINE-DOBBERNACK E.

Summary

Plant cells are especially difficult to cryopreserve. The reason can be seen in the high water content and the sub-cellular structure of these cells containing vacuoles filled with water. Nevertheless different technical approaches exist today to achieve a mild dehydration of plant cells avoiding intracellular ice crystal formation or achieving vitrification of the protoplast during exposure to ultra low temperatures. For most dedifferentiated cell cultures still “slow freezing” is applied. One of the cell cultures used most often for applications in fundamental research is the BY-2 cell line. This cell culture has been initiated from the Nicotiana tabacum variety “Bright Yellow 2”. The cell line has also been preserved by “slow freezing” approaches. Recently we developed another approach based on vitrification/encapsulation and combined it with ultra-rapid freezing. Although the method yielded positive results, there is still no professional equipment to guarantee a reproducible and safe application of the method.

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Pages: 154-158

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Details

  • Original title: A preliminary method for ultra-rapid freezing of the Nicotiana tabacum BY-2 cell line by encapsulation/vitrification.
  • Record ID : 30005951
  • Languages: English
  • Source: Cryogenics 2012. Proceedings of the 12th IIR International Conference: Dresden, Germany, September 11-14, 2012.
  • Publication date: 2012/09/11

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