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Characterization of a protease from a psychrotroph, Pseudomonas fluorescens 114.

Author(s) : HAMAMOTO T., KANEDA M., HORIKOSHI K., KUDO T.

Type of article: Article

Summary

A psychrotrophic bacterium isolated from river sediment was identified as Pseudomonas fluorescens 114. It grew at 0 and optimally at 20 deg C. The bacterium produced a protease with a molecular weight of 47,000, which was stable in the pH range of 5 to 9 and worked optimally between pH 6.5 and 10. Activity was optimal at 35 deg C and was lost immediately at 50 deg C and after 5 minutes at 45 deg C. At 0, 10, and 20 deg C, 24, 38, and 57% of optimal activity were observed, respectively.

Details

  • Original title: Characterization of a protease from a psychrotroph, Pseudomonas fluorescens 114.
  • Record ID : 1995-3231
  • Languages: English
  • Source: Appl. environ. Microbiol. - vol. 60 - n. 10
  • Publication date: 1994/10
  • Document available for consultation in the library of the IIR headquarters only.

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