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CRYOPRESERVATION OF HUMAN PLATELETS WITH HYDROXYETHYL STARCH IN A ONE-STEP PROCEDURE.

Author(s) : SPUTTEK A.

Type of article: Article

Summary

THE RESULTS OF MORE THAN 300 FREEZING EXPERIMENTS ALLOWED TO DETERMINE THE RANGE OF OPTIMAL COOLING RATES AS BEING 0.08 TO 0.25 K/S, WHEN AN ISOTONIC MILIEU AND A STARCH CONCENTRATIONOF 3.6% WERE APPLIED. HES 40/0.5 AND HES 200/0.5 SHOWED ALMOST IDENTICAL RESULTS AND WERE SUPERIOR TO HES 450/0.6. A SURVIVAL OF ALMOST 90% WAS ACCOMPANIED BY AN INCREASE IN VOLUME BY ABOUT 50% AND A DROP TO LESS THAN 30% OF THE INTRACELLULAR ATP-LEVEL. ALTHOUGH THE THERAPEUTIC QUALITY OF CRYOPRESERVED PLATELETS CAN BE ASSESSED ONLY BY IN VIVO INVESTIGATIONS, THE ESTIMATION OF PHYSIOLOGICAL FUNCTION BY RESONANCE THROMBOGRAPHY AMOUNTED TO NEARLY 80%.

Details

  • Original title: CRYOPRESERVATION OF HUMAN PLATELETS WITH HYDROXYETHYL STARCH IN A ONE-STEP PROCEDURE.
  • Record ID : 1988-0394
  • Languages: English
  • Source: Cryo-Letters - vol. 8 - n. 4
  • Publication date: 1987
  • Document available for consultation in the library of the IIR headquarters only.

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