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Successful cryopreservation of fetal porcine proislets.

Author(s) : LIU X., BRENDEL M. D., KLITSCHER D., BRANDHORST H., HERING B. J., FEDERLIN K. F., BRETZEL R. G.

Type of article: Article

Summary

The study reports on the cryopreservation of purified fetal pig proislets which were isolated by a culture technique. The fetal pig proislets were equilibrated with 2 M dimethyl sulfoxide, cooled at a rate of 0.25 deg C/min to -40 deg C, transferred to liquid nitrogen (-196 deg C), stored from 2 hours to 2 months at -196 deg C, and thawed in a 37 deg C water bath. The morphology of frozen-thawed fetal pig proislets was similar to that of non-cryopreserved fetal pig proislets. One hundred percent recovery of response capacity was achieved when tested with 16.7 milliM glucose plus 10 milliM theophylline.

Details

  • Original title: Successful cryopreservation of fetal porcine proislets.
  • Record ID : 1994-1878
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 3
  • Publication date: 1993/06

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