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Cryopreservation of carrot somatic embryos using a simplified freezing process.

Author(s) : LECOUTEUX C., et al.

Type of article: Article

Summary

Heart and torpedo-shaped somatic embryos of carrot (Daucus carota L.) were cryopreserved after a pretreatment of 24 hours in a basal liquid medium supplemented with 0.4 M sucrose as the sole cryoprotectant. The embryos were then cooled by placing in a domestic freezer at -20 deg C for 24 hours, prior to immersion in liquid nitrogen. 80% of the embryos survived after thawing and developed further into plantlets with the same frequencies as unfrozen control embryos. In these conditions, survival was not the result of secondary embryogenesis or embryogenic callus formation producing multiple-shooted plantlets. It was the expression of a direct regrowth of whole somatic embryos.

Details

  • Original title: Cryopreservation of carrot somatic embryos using a simplified freezing process.
  • Record ID : 1993-0265
  • Languages: English
  • Source: Cryo-Letters - vol. 12 - n. 6
  • Publication date: 1991/11
  • Available in the IIR library

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