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DROPLET FREEZING OF ANTIBODY-LINKED INDICATOR RED CELLS OF SHEEP, OX AND HUMAN ORIGIN.

Author(s) : PEGG D. E.

Type of article: Article

Summary

A DROPLET FREEZING TECHNIQUE FOR THE CRYOPRESERVATION OF INDICATOR RED CELLS IS DESCRIBED. RECOVERY WAS CRUCIALLY DEPENDENT ON THE COMPOSITION OF THE SOLUTION IN WHICH THE CELLS WERE SUSPENDED. IT WAS SHOWN THAT GLUCOSE PENETRATED THE CELLS WHEREAS SUCROSE DID NOT. TEN DIFFERENT ANTIBODY-COUPLED REAGENT CELLS ALL GAVE MORE THAN 83% RECOVERY. THE PRESERVATION TECHNIQUE DESCRIBED IS SIMPLE AND CONVENIENT, AND WILL MAKE IT POSSIBLE TO EXTEND THE USE OF IMMUNOASSAY PROCEDURES USING ANTIBODY-COUPLED RED CELLS.

Details

  • Original title: DROPLET FREEZING OF ANTIBODY-LINKED INDICATOR RED CELLS OF SHEEP, OX AND HUMAN ORIGIN.
  • Record ID : 1983-2004
  • Languages: English
  • Source: Cryobiology - vol. 19 - n. 6
  • Publication date: 1982

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