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Cryopreservation of fish spermatozoa: effect of cooling methods on the reproducibility of cooling rates and viability.

Author(s) : RANA K. J., GILMOUR A.

Summary

Although successful cryopreservation of fish egg and embryos remains elusive the preservation spermatozoa is currently feasible and practical. The reproducibility of most current protocols for fish spermatozoa preservation, however, is unsatisfactory and commercial uptake of low temperature technology for gamete preservation is low. Varying levels of cryosuccess has been reported for spermatozoa from up to 200 fish species, notably within the salmonids, tilapias and carps. The overall success and efficacy of the cryopreservation protocols will vary depending on interrelated variables such as milt quality, precooling storage conditions, packaging, cooling methods and rates, post cooling storage conditions, warming regimes and management of post-thawed milt. The paper discusses the variability of cooling rates generated by different cooling methods. Cooling rates between samples can vary by as much as 500% within a single trial. By optimising the above factors, using specially designed large straws and controlled rate cooling fertility rates similar to control values have been achieved in large scale salmonid cryopreservation trials using between 1500-6000 eggs/treatment. The results from some of these studies and a unified field tilapia cryopreservation system are presented.

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Details

  • Original title: Cryopreservation of fish spermatozoa: effect of cooling methods on the reproducibility of cooling rates and viability.
  • Record ID : 1997-3643
  • Languages: English
  • Source: Refrigeration and Aquaculture.
  • Publication date: 1996/03/20
  • Document available for consultation in the library of the IIR headquarters only.

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