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Cryopreservation of reproductive elements of macroalgae.

Cryoconservation des éléments reproducteurs de macroalgues.

Author(s) : ARBAULT S., KAAS R.

Summary

To avoid maintenance problems during cultivation of reproductive cells of macroalgae, deep freezing at -196 deg C was used. Cryopreservation techniques were developed on gametophytes and spores of Undaria pinnatifida and on the conchocelis phase of Porphyra linearis. It consists in an immersion in a cryoprotectant, then a slow decrease in temperature down to -40 deg C, followed by a rapid immersion in liquid nitrogen. After quick thawing, restarting of new cultures were tested. For each species and even for each stage in the life cycle of an alga the technique has to be adapted. For Undaria pinnatifida the cryoprotectant was glycerol and, after thawing, zygotes were obtained after 7 days of culture. In the case of Porphyra linearis two cryoprotectants were effective: dimethylsulfoxide and saccharose. After thawing, cells recovered after a period of 14 to 21 days. These innovative studies emphasize the necessity of adapting each time the whole deep freezing process to each kind of cell.

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Details

  • Original title: Cryoconservation des éléments reproducteurs de macroalgues.
  • Record ID : 1997-0967
  • Languages: French
  • Source: Refrigeration and Aquaculture.
  • Publication date: 1996/03/20
  • Document available for consultation in the library of the IIR headquarters only.

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