Cryopreservation of tea (Camellia sinensis L.) seeds and embryonic axes.

Author(s) : KIM H. H., CHA Y. S., BAEK H. J., et al.

Type of article: Article


The authors investigated the tolerance to desiccation and freezing of tea seeds, embryonic axes (EAs) and cotyledonary embryonic axes (CEAs). No seeds germinated after desiccation and cryopreservation. EAs extracted from seeds desiccated to 18.9% moisture content and cryopreserved showed 20.7% survival but plantlet production was impossible. When EAs and CEAs were extracted from seeds before desiccation and freezing, survival of control and frozen samples was equivalent with both types of materials. However, plantlet production was significantly higher from control and cryopreserved CEAs than EAs. The maturity stage of the seeds from which CEAs were extracted had an important effect on survival and plant production, mature seeds providing better results than early mature and late mature seeds. The highest percentages of plantlet production from cryopreserved CEAs (75.1 and 80.4%) were achieved for EA moisture contents of 21.5-15.0%.


  • Original title: Cryopreservation of tea (Camellia sinensis L.) seeds and embryonic axes.
  • Record ID : 2003-1873
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 4
  • Publication date: 2002/07
  • Document available for consultation in the library of the IIR headquarters only.


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