ShareCryopreservation of yam (Dioscorea spp.) shoot apices by encapsulation-dehydration.
Author(s) : MANDAL B. B., CHANDEL K. P. S., DWIVEDI S.
Type of article: Article
Summary
Shoot apices of in vitro grown yam were cryopreserved using the encapsulation-dehydration technique. Maximum survival of frozen apices (up to 71%) was obtained when alginate-coated apices were precultured for 3 days in liquid medium with 0.75 M sucrose followed by 4 hours of desiccation in sterile air-flow, rapid freezing and slow thawing.
Details
- Original title: Cryopreservation of yam (Dioscorea spp.) shoot apices by encapsulation-dehydration.
- Record ID : 1997-0837
- Languages: English
- Source: Cryo-Letters - vol. 17 - n. 3
- Publication date: 1996/05
- Document available for consultation in the library of the IIR headquarters only.
Links
See other articles in this issue (8)
See the source
Indexing
- Themes: Seeds and plants
- Keywords: Cryobiology; Survival; Sucrose; Shoot; Yam; Dehydration; Cryopreservation
-
Cryopreservation of apple shoot tips by encapsu...
- Author(s) : ZHAO Y., WU Y., ENGELMANN F., ZHOU M., ZHANG D., CHEN S.
- Date : 1999/03
- Languages : English
- Source: Cryo-Letters - vol. 20 - n. 2
View record
-
Effect of pretreatment conditions on the cryopr...
- Author(s) : MALAURIE B., TROUSLOT M. F., ENGELMANN F., CHABRILLANGE N.
- Date : 1998/01
- Languages : English
- Source: Cryo-Letters - vol. 19 - n. 1
View record
-
INFLUENCE OF DAYS OF CULTURE ON CRYOPROTECTANT-...
- Author(s) : MCADAMS S., RATNASABAPATHI D., SMITH R. A.
- Date : 1991/06
- Languages : English
- Source: Cryobiology - vol. 28 - n. 3
View record
-
Importance of pregrowth with sucrose and vitrif...
- Author(s) : GONZALEZ ARNAO M. T., MOREIRA T., URRA C.
- Date : 1996/05
- Languages : English
- Source: Cryo-Letters - vol. 17 - n. 3
View record
-
Cryopreservation of Vitis vinifera L. cv Chardo...
- Author(s) : PLESSIS P., LEDDET C., COLLAS A., DEREUDDRE J.
- Date : 1993/09
- Languages : English
- Source: Cryo-Letters - vol. 14 - n. 5
View record