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Cryopreservation of Vitis vinifera L. cv Chardonnay shoot tips by encapsulation-dehydration: effects of pretreatment, cooling and postculture conditions.

Author(s) : PLESSIS P., LEDDET C., COLLAS A., DEREUDDRE J.

Type of article: Article

Summary

Optimal pretreatments, freezing and postculture conditions were studied for grapevine shoot tips cryopreserved by the encapsulation-dehydration process. Best results (40-60% survival and 30% shoot recovery without callus formation) were obtained after a 2-day stepwise preculture in a medium containing 1 M sucrose, two-step cooling, and direct subculture on standard culture medium. Higher survival rates were noted at lower precooling temperatures and cooling rates. Tolerance of shoot tips was independent of their position on the stem.

Details

  • Original title: Cryopreservation of Vitis vinifera L. cv Chardonnay shoot tips by encapsulation-dehydration: effects of pretreatment, cooling and postculture conditions.
  • Record ID : 1994-1624
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 5
  • Publication date: 1993/09
  • Document available for consultation in the library of the IIR headquarters only.

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