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DECLINED VIABILITY AND DIFFERENTIATION OF HUMAN BONE MARROW STEM CELLS BY AGING, CRYOPRESERVATION AND ADVANCED SOLID CANCER.

Author(s) : MATSUKI R.

Type of article: Article

Summary

AN EQUAL VOLUME OF A 20% DMSO IN TC199 AND AUTOLOGOUS PLASMA WAS ADDED TO THE CONCENTRATED CELL SUSPENSION IN A SPECIAL PLASTIC BAG AND FROZEN AT 2 K PER MINUTE IN A LIQUID NITROGEN FREEZER AND STORED IN LIQUID NITROGEN IN EXCESS OF FIVE YEARS. THAWED SAMPLES AT 313 K (40 DEG C) WERE DILUTED WITH TC199, WASHED, RESUSPENDED AND ASSAYED WITH THE METHYLCELLULOSE TECHNIQUE DESCRIBED BY ISCOVE (1971). MARROW CELLS FROM 75 PATIENTS SHOWED A 0.03-0. 09% CFU-G SURVIVAL OF THE FREEZING PROCESS, BUT THE RECOVERY RATE OF CFU-G WAS IMPAIRED DURING 5 YEARS STORAGE. A ZERO RECOVERY AFTER 92 MONTHS IS ASSUMED. THE MARROW CELLS OF CANCER OF THE ALIMENTARY TRACTS INDICATED A LOWER CFU RECOVERY THAN THOSE OF OTHER CANCERS. G.R.S.

Details

  • Original title: DECLINED VIABILITY AND DIFFERENTIATION OF HUMAN BONE MARROW STEM CELLS BY AGING, CRYOPRESERVATION AND ADVANCED SOLID CANCER.
  • Record ID : 1990-0849
  • Languages: English
  • Source: Low Temperature Medicine - vol. 15 - n. 1
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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