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DEVELOPMENT OF A MINIATURISED CRYOPRESERVATION METHOD FOR THE MAINTENANCE OF A WIDE RANGE OF YEASTS.

Author(s) : KIRSOP B., HENRY J.

Type of article: Article

Summary

USING AEROBICALLY GROWN, STATIONARY PHASE CELLS AND 5% GLYCEROL AS A CRYOPROTECTANT A 2-STEP COOLING METHOD HAS BEEN ESTABLISHED FOR THE CRYOPRESERVATION OF A WIDE RANGE OF YEASTS. SURVIVAL LEVELS ARE HIGH AND STRAINS APPEAR STABLE. RESPIRATORY DEFICIENT MUTANTS WERE NOT DETECTED AND PLASMIDS WERE NOT LOST DURING FREEZING AND THAWING.

Details

  • Original title: DEVELOPMENT OF A MINIATURISED CRYOPRESERVATION METHOD FOR THE MAINTENANCE OF A WIDE RANGE OF YEASTS.
  • Record ID : 1985-0811
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 3
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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