Effect of freezing and thawing on the viability and uptake of amino acids by Lactobacillus delbrueckii ssp. bulgaricus.
Author(s) : VALDEZ G. F. de, GIORI G. S. de
Type of article: Article
Summary
Freezing and thawing damaged the cell membrane of Lactobacillus delbrueckii ssp. bulgaricus, thus rendering the microorganism sensitive to sodium chloride and liver extract. These substances had no inhibitory effect on the unfrozen cells. The amino acids transport system was also depressed in all the freezing regimens tested, a fact that indicates the existence of a close relationship between this system and the viability of the cells. Cell injury proved reversible and could be partially repaired with a solution of KH(2)PO(4), pyruvate, and sulphate.
Details
- Original title: Effect of freezing and thawing on the viability and uptake of amino acids by Lactobacillus delbrueckii ssp. bulgaricus.
- Record ID : 1994-1865
- Languages: English
- Source: Cryobiology - vol. 30 - n. 3
- Publication date: 1993/06
Links
See other articles in this issue (10)
See the source
Indexing
-
Protective effects of amino acids against freez...
- Author(s) : KRUUV J., GLOFCHESKI D. J.
- Date : 1992/04
- Languages : English
- Source: Cryobiology - vol. 29 - n. 2
View record
-
Improvement of motility and fertilization poten...
- Author(s) : RENARD P., GRIZARD G., CRIVEAU J. F., SION B., BOUCHER D., LE LANNOU D.
- Date : 1996/06
- Languages : English
- Source: Cryobiology - vol. 33 - n. 3
View record
-
Interactions of proline, serine, and leucine wi...
- Author(s) : POPOVA A. V., SCHMITT J. M., HINCHA D. K.
- Date : 1998/08
- Languages : English
- Source: Cryobiology - vol. 37 - n. 1
View record
-
MECHANISM OF IMPAIRMENT OF AMINO ACID TRANSPORT...
- Author(s) : GULEVSKIJ A. K.
- Date : 1990
- Languages : English
- Source: Cryo-Letters - vol. 11 - n. 6
View record
-
THE EFFECT OF CRYOPRESERVATION ON THE TRANSPORT...
- Author(s) : GRISCENKOVA E. A., GULEVSKIJ A. K., RJAZANCEV V. V.
- Date : 1989
- Languages : Russian
- Source: Kriobiologija - n. 2
View record