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EFFECT OF HYPERTONICITY ON SURVIVAL OF UNPROTECTED HUMAN CULTURED CELLS FOLLOWING FREEZING AND THAWING.

Author(s) : TENCHINI M. L., BOLOGNANI L., CARLI L. de

Type of article: Article

Summary

AN INVESTIGATION WAS CARRIED OUT ON THE POST-THAW SURVIVAL OF UNPROTECTED HUMAN HETEROPLOID EUE (HUMAN EMBRYONIC EPITHELIUM) CELLS, EITHER MAINTAINED IN ISOTONIC MEDIUM (0.137 M NACL) OR ADAPTED TO HYPERTONICITY (0.356 M NACL) AND FROZEN IN MEDIUM WITH AN INCREASED CONCENTRATION OF NACL. A FIVEFOLD INCREASE IN THE SURVIVAL FRACTION OF THE ADAPTED CELLS IN COMPARISON WITH THE UNADAPTED ONES WAS OBSERVED WHEN CELLS WERE FROZEN IN ISOTONIC MEDIUM. IN HYPERTONIC MEDIUM, THE TWO CELL TYPES EXHIBIT COMPARABLE SURVIVAL VALUES.

Details

  • Original title: EFFECT OF HYPERTONICITY ON SURVIVAL OF UNPROTECTED HUMAN CULTURED CELLS FOLLOWING FREEZING AND THAWING.
  • Record ID : 1981-0277
  • Languages: English
  • Source: Cryobiology - vol. 17 - n. 2
  • Publication date: 1980/04

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