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The effect of temperature at which slow cooling is terminated and of thawing rate on the survival of one-cell mouse embryos frozen in dimethyl sulfoxide or 1,2-propanediol solutions.

Author(s) : ABBEEL E. van den, ELST J. van der, STEIRTEGHEM A. C. van

Type of article: Article

Summary

The results indicate that high rates of survival may be obtained from one-cell mouse embryos by a rapid or a slow thawing procedure, as has been found for other developmental stages. The results also indicate that 1,2-propanediol provides superior protection compared to DMSO against freezing-thawing damage and that the addition of sucrose to the freezing solutions prior to freezing improves the overall survival rates.

Details

  • Original title: The effect of temperature at which slow cooling is terminated and of thawing rate on the survival of one-cell mouse embryos frozen in dimethyl sulfoxide or 1,2-propanediol solutions.
  • Record ID : 1995-1869
  • Languages: English
  • Source: Cryobiology - vol. 31 - n. 5
  • Publication date: 1994/10

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