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COMPOUNDS WHICH PROTECT FREEZE-DRIED CULTURES FROM MUTATION.

Author(s) : IIJIMA T.

Summary

A FREEZE-DRYING METHOD (ANNEAR 1970) HAS BEEN APPLIED TO MAINTAIN BACTERIA AND YEASTS IN CULTURE COLLECTIONS. WHEN THE FREEZE-DRIED SPECIMENS WERE KEPT AT A HIGH TEMPERATURE, THE NUMBER OF MUTANT CELLS WAS INCREASED IN EXPERIMENTS USING ESCHERICHIA COLI. ADDITION OF ADONITOL, CYSTEIN OR THIOUREA RAISED SURVIVING RATIO OF RECA MUTANT AND LESSENED THE REVERSION OF A TRP REC + A MUTANT. WHEN THE CELLS WERE DRIED IN POTASSIUM PHOSPHATE BUFFER CONTAINING MONOSODIUM GLUTAMATE, NO DNA SINGLE-STRAND BREAKS WERE DETECTED AFTER THE DRYING. PROTECTIVE SUBSTANCES RESULTED IN NO SIGNIFICANT DNA BREAKS AFTER DRYING AND AFTER PRESERVATION AT LOW TEMPERATURE. DNA BREAKS IN THE CELLS DRIED WITH THE PROTECTANTS DECREASED AS COMPARED WITH THOSE WITHOUT PROTECTANTS.

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Details

  • Original title: COMPOUNDS WHICH PROTECT FREEZE-DRIED CULTURES FROM MUTATION.
  • Record ID : 1987-1213
  • Languages: English
  • Source: Fundamentals and Applications of Freeze-Drying to Biological Materials, Drugs and Foodstuffs.
  • Publication date: 1985/05/20
  • Document available for consultation in the library of the IIR headquarters only.

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