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Freezing preservation of the mammalian cardiac explant. 2. Comparing the protective effect of glycerol and polyethylene glycol.

Author(s) : BANKER M. C., LAYNE J. R. Jr, HICKS G. L. Jr, et al.

Type of article: Article

Summary

The authors compared the cryoprotective ability of glycerol and polyethylene glycol (PEG) during freezing. Isolated rat hearts were flushed with one of three cardioplegic solutions (CP-14, CP-15, and CP-16), frozen at -1.4 deg C, and reperfused after thawing to assess function. The results of comparison are given for the different cardioplegic solutions, and for different times of freezing. Evolution of adenosine 5'-monophosphate, adenosine 5'-diphosphate and adenosine 5'-triphosphate and total adenine nucleotide (TAN) are discussed. In conclusion, both glycerol and PEG offered cryoprotection by reducing tissue ice formation. PEG was superior by reducing tissue ice content further via dehydration and by better preserving TAN content.

Details

  • Original title: Freezing preservation of the mammalian cardiac explant. 2. Comparing the protective effect of glycerol and polyethylene glycol.
  • Record ID : 1993-0535
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 1
  • Publication date: 1992/02

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