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Fundamental study for cancer cryoablation.

[In Japanese. / En japonais.]

Author(s) : PARK M., TERADA T., AKIYAMA Y., et al.

Type of article: Article

Summary

To examine the mechanism of cell death, it is important to design a temperature programme for cryoablation. In this study, the cause of cell death is discussed based on the correlation between cell survival rate and cell morphology. Cell survival rate after freezing and thawing was evaluated using the MTT [3-(4,5-dimethyl-2-thiazoly)-2,5-diphenyltetrazolium bromide] method. The authors observed a change in cell morphology during the freezing-thawing process using an optical microscope. The survival rate decreased, and the percentage of cell diameter change rate increased as the freezing-thawing speed increased. This result suggests that a large change in the ratio of cell diameter causes cell death. It is believed that the osmosis phenomenon during freezing and thawing is one of the factors that causes changes in cell morphology and it results in the cell death.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 2008-1388
  • Languages: Japanese
  • Source: Journal of the Cryogenic Society of Japan - vol. 42 - n. 8
  • Publication date: 2007

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