Share

Influence of cold stress on the preliminary enrichment time needed for detection of enterohemorrhagic Escherichia coli in ground beef by PCR.

Author(s) : UYTTENDAELE M., GRANGETTE C., ROGERIE F., PASTEAU S., DEBEVERE J., LANGE M.

Type of article: Article

Summary

When cells are exposed to cold stress (4 or -20 deg C), a 24-hour enrichment period is recommended. Restriction of enrichment time to 9 hours under these circumstances decreases the sensitivity of PCR detection to 80 CFU/gramme. Hence, to obtain maximum sensitivity, PCR detection of VTEC (verotoxin-producing E. coli) in naturally contaminated beef should be performed after 24 hours of enrichment.

Details

  • Original title: Influence of cold stress on the preliminary enrichment time needed for detection of enterohemorrhagic Escherichia coli in ground beef by PCR.
  • Record ID : 1999-0905
  • Languages: English
  • Source: Appl. environ. Microbiol. - vol. 64 - n. 5
  • Publication date: 1998/05

Links


See other articles in this issue (1)
See the source

Indexing