Share

INFLUENCE OF FREEZE-DRYING OVER PRESERVATION OF THE ENZYME ACTIVITY OF HUMICOLA LUTEA 120-5 MOULD MUTAGEN STRAIN.

[In Bulgarian. / En bulgare.]

Author(s) : IVANOVA L., ALEKSIEVA I. P.

Summary

THE INFLUENCE OF FREEZE-DRYING OVER THE ENZYME ACTIVITY OF HUMICOLA LUTEA 120-5 MOULD MUTAGEN STRAIN -PRODUCER OF ACID PROTEASE -WAS STUDIED. IT WAS FOUND OUT THAT OF THE 5 EXPERIMENTED PROTECTIVE MEDIA THE MOST SUITABLE ONE FOR ITS FREEZE-DRYING WAS THE MODIFIED GLUTAMIC-DEXTRAN-SUCRASE MEDIUM (WITH A RATE OF ITS COMPONENTS 1:1:2) PROVIDING 100% SURVIVAL OF THE SPORES. THE OPTIMAL STORAGE TEMPERATURE ENSURING THE CONDITION OF FREEZE-DRYING WAS 277 K(4 DEG C). AFTER A PERIOD OF 8 YEARS STORAGE OF H. LUTEA 120-5 STRAIN UNDER THIS TEMPERATURE, THE AUTHORS OBSERVED MORPHOLOGICAL STABILITY AND 100% PRESERVATION OF THE LEVEL OF ITS PROTEOLYTIC ACTIVITY.

Details

  • Original title: [In Bulgarian. / En bulgare.]
  • Record ID : 1988-2114
  • Languages: Bulgarian
  • Publication date: 1987/08/02
  • Source: Source: Proc. 3rd natl. Sch. Cryobiol. Freeze-drying, Sofia
    259-262; 2 fig.; 1 tabl.; 11 ref.
  • Document available for consultation in the library of the IIR headquarters only.

Indexing