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INVESTIGATION INTO THE EFFICIENCY OF PEG-400 AND DMSO UPON THE CRYOPRESERVATION OF ISOLATED HEPATOCYTES.

[In Russian. / En russe.]

Author(s) : SEMENCHENKO O. A., KRAVCHENKO L. P., LUGOVOJ V. I.

Type of article: Article

Summary

CRYOPROTECTANTS IN A FINAL 10% CONCENTRATION USED IN SALT SOLUTIONS (VIZ 0. 15 M SODIUM CHLORIDE, KREBS-RINGER BUFFER, HANK'S SOLUTION, ALBUMIN MEDIUM OR MEDIUM-199) FAVOUR THE PRESERVATION OF ISOLATED HEPATOCYTES. THIS HAS BEEN PROVEN BY DECREASING THE LIBERATION OF POTASSIUM IONS AND ENZYME PROTEINS INTO THE FREEZING MEDIUM. SOLUTIONS OF 10% DMSO WERE FOUND TO BE MORE EFFECTIVE IN THE PROTECTION OF THE CELLS FROM FREEZING INJURY AS COMPARED WITH 10% PEG-400.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1989-0356
  • Languages: Russian
  • Source: Kriobiologija - n. 2
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

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