LIPOAMIDE DEHYDROGENASE, CITRATE SYNTHASE, AND BETA-HYDROXYACYL-COENZYME A-DEHYDROGENASE IN SKELETAL MUSCLE. 8. INFLUENCE OF TEMPERATURE AND RATE OF FREEZING OF BOVINE MUSCLE ON ACTIVITY AND SUBCELLULAR DISTRIBUTION OF THE ENZYMES IN THE THAWED TISSUE.

[In German. / En allemand.]

Author(s) : HAMM R., GOTTESMANN P.

Type of article: Article

Summary

SAMPLES OF BOVINE MUSCLE (POSTRIGOR) WERE FROZEN AT DIFFERENT TEMPERATURES BETWEEN -5 AND -196 DEG C AT DIFFERENT FREEZING RATES, AND THAWED AT ROOM TEMPERATURE. NEITHER THE TEMPERATURE NOR THE RATE OF FREEZING SHOWED A SIGNIFICANT INFLUENCE ON THE TOTAL ENZYME ACTIVITIES. BELOW -10 DEG C THE RELEASE OF ENZYMES INTO THE SARCOPLASMIC FLUID INCREASED UPON RAPID FREEZING. RAPID FREEZING BELOW -20 DEG C RESULTED IN A SIGNIFICANTLY STRONGER RELEASE OF ENZYMES THAN SLOW FREEZING. DAMAGE TO MITOCHONDRIAL MEMBRANES UPON FAST FREEZING IS PRIMARILY A RESULT OF INTRACELLULAR ICE FORMATION, WHEREAS THE MEMBRANE DAMAGE DURING SLOW FREEZING IS PRIMARILY DUE TO DEHYDRATION.

Details

  • Original title: [In German. / En allemand.]
  • Record ID : 1986-1506
  • Languages: German
  • Source: Z. Lebensm.-Unters. -Forsch. - n. 181
  • Publication date: 1985
  • Document available for consultation in the library of the IIR headquarters only.

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