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LYMNAEA STAGNALIS L BRAIN GIANT NEURONS PRESERVE THEIR VIABILITY AFTER DEEP FREEZING.

[In Russian. / En russe.]

Author(s) : GAHOVA E. N.

Type of article: Article

Summary

SAMPLES OF ISOLATED BRAIN FROM SNAILS LYMNAEA STAGNALIS L WERE FROZEN IN LIQUID NITROGEN VAPOUR AT A RATE OF 500 K/MIN IN THE PRESENCE OF 1.5 M DMSO, ETHYLENE GLYCOL OR FORMAMIDE. THE SAMPLES WERE THEN PLUNGED INTO LIQUID NITROGEN AND STORED FOR UP TO 2 MONTHS. THAWING WAS PERFORMED IN A WATER BATH AT 296 TO 298 K (23 TO 25 DEG C). TWO-THREE HOURS AFTER THAWING AND SUBSEQUENT INCUBATION IN PHYSIOLOGICAL SOLUTION, THE ELECTRICAL PARAMETERS FOR ISOLATED GIANT NEURONS (150 TO 200 MICROMETERS IN SIZE) WERE SIMILAR TO THOSE OF CONTROL ONES.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1990-0414
  • Languages: Russian
  • Source: Kriobiologija - n. 1
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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