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Mechanisms of dehydration injury in bacteria.

Author(s) : ASHWOOD-SMITH M. J.

Type of article: Article, IJR article

Summary

Genetic injuries caused by freeze-drying and desiccation are discussed. The experiments clearly demonstrate that the process of freeze-drying causes a variety of mutations in bacteria. The number of mutations increases as a function of the duration of cold storage. Breaks in DNA single strands were produced and, immediately after freeze-drying, the activation and formation of lambda bacteriophages from lysogenic bacteria was observed. It is clear that cryopreservation is better than freeze-drying for the long term storage of microorganisms when one requires absolute genetic stability.

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Format PDF

Pages: 205-212

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Details

  • Original title: Mechanisms of dehydration injury in bacteria.
  • Record ID : 30000898
  • Languages: English
  • Source: International Journal of Refrigeration - Revue Internationale du Froid - vol. 3 - n. 4
  • Publication date: 1980/07

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