ShareMODES OF INTERACTION OF CRYOPROTECTANTS WITH MEMBRANE PHOSPHOLIPIDS DURING FREEZING.
Author(s) : ANCHORDOGUY T. J.
Type of article: Article
Summary
THE ABILITIES OF A VARIETY OF COMPOUNDS TO INHIBIT LIPOSOME FUSION DURING FREEZE/THAW WERE ASSESSED BY RESONANCE ENERGY TRANSFER. SMALL UNILAMELLAR VESICLES HAVE BEEN FROZEN ACCORDING TO 3 DIFFERENT PROTOCOLS. MEMBRANE INTERMIXING WAS SEEN TO BE RELATIVELY INDEPENDENT OF FREEZING PROTOCOL EXCEPT WHEN GLYCEROL, DMSO, OR SARCOSINE WAS USED AS THE CRYOPROTECTANT. THE MOST EFFECTIVE CRYOPROTECTANTS WERE TREHALOSE AND SUCROSE, WHICH BOTH REDUCED FUSION TO MINIMAL LEVELS AT CONCENTRATIONS OF ONLY 0.2 M.
Details
- Original title: MODES OF INTERACTION OF CRYOPROTECTANTS WITH MEMBRANE PHOSPHOLIPIDS DURING FREEZING.
- Record ID : 1989-0344
- Languages: English
- Source: Cryobiology - vol. 24 - n. 4
- Publication date: 1987/08
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cryoprotectant; Cryobiology; Cell; DMSO; Sucrose; Phospholipid; Membrane; Freezing
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- Date : 1981
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- Author(s) : MORRIS G. J., MCGRATH J. J.
- Date : 1981/08
- Languages : English
- Source: Cryobiology - vol. 18 - n. 4
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