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THE EFFECT OF DMSO, SUCROSE OR VARIOUS SALT SOLUTIONS ON THE SURVIVAL OF CELLS EXPOSED TO 273 K (O.C) OR FROZEN TO 77 K (-196.C).

Author(s) : RAAPHORST G. P.

Type of article: Article

Summary

CELLS, FROZEN TO 77 K (-196.C) OR EXPOSED TO 273 K (0.C) TEMPERATURE SHOCK, AS A FUNCTION OF MOLARITY OF VARIOUS NEUTRAL SALT SOLUTIONS HAD DIFFERENT MAXIMA IN SURVIVAL WITH RESPECT TO MOLARITY AND RELATIVE SURVIVAL. THESE DIFFERENCES COULD NOT BE ACCOUNTED FOR BY DIFFERENCES IN CELL VOLUME OR WATER PERMEABILITY RATE DURING THE COOLING PROCESS. THE ION SPECIFICITY BETWEEN CACL2 AND THE MONOVALENT ALKALI METAL SALTS WAS SIGNIFICANT. NO CORRELATION BETWEEN CELL WATER STRUCTURE, AND CELL SURVIVAL IN THE VARIOUS SALT, SUCROSE OR DMSO SOLUTIONS WAS FOUND.

Details

  • Original title: THE EFFECT OF DMSO, SUCROSE OR VARIOUS SALT SOLUTIONS ON THE SURVIVAL OF CELLS EXPOSED TO 273 K (O.C) OR FROZEN TO 77 K (-196.C).
  • Record ID : 1982-0286
  • Languages: English
  • Source: Cryo-Letters - vol. 2 - n. 5
  • Publication date: 1981
  • Document available for consultation in the library of the IIR headquarters only.

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