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ROLES OF UNFROZEN FRACTION, SALT CONCENTRATION AND CHANGES IN CELL VOLUME IN THE SURVIVAL OF FROZEN HUMAN ERYTHROCYTES.

Author(s) : MAZUR P., COLE K. W.

Type of article: Article

Summary

DURING SLOW FREEZING, CELLS ARE SEQUESTERED IN UNFROZEN CHANNE LS BETWEEN ICE CRYSTALS THAT GROW BY REMOVING PURE WATER FROM THE CHANNELS. AS A CONSEQUENCE, THE SOLUTE CONCENTRATION IN THE CHANNELS RISES AND THE VOLUME OF LIQUID IN THE CHANNELS PROGRESSIVELY DECREASES. THE RISE IN SOLUTE CONCENTRATION, IN TURN, CAUSES THE CELLS TO PROGRESSIVELY SHRINK OSMOTICALLY. THE AUTHORS EXAMINED AND COMPARED THE EFFECT OF RETURNING THE THAWED CELLS TO ISOTONIC SOLUTIONS AND ISOTONIC VOLUME.

Details

  • Original title: ROLES OF UNFROZEN FRACTION, SALT CONCENTRATION AND CHANGES IN CELL VOLUME IN THE SURVIVAL OF FROZEN HUMAN ERYTHROCYTES.
  • Record ID : 1990-0410
  • Languages: English
  • Source: Cryobiology - vol. 26 - n. 1
  • Publication date: 1989

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