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STUDIES ON CRYOPRESERVATION OF HAMSTER PANCREATIC ISLETS USING A FAIRLY RAPID COOLING RATE. THE VIABILITIES OF CRYOPRESERVED PANCREATIC ISLETS.

[In Japanese. / En japonais.]

Author(s) : FUKUSHIMA W.

Type of article: Article

Summary

CONCERNING THE USE OF CRYOPRESERVATION FOR PANCREATIC ISLET STORAGE, THERE ARE SEVERAL FACTORS TO CONSIDER IN ORDER TO OBTAIN THE BEST FUNCTIONAL PRESERVATION OF ISLET CELLS. THE MOST OBVIOUS ONE IS THE COOLING RATE. IN THE STUDY, THE ISOLATED ISLETS WERE CULTURED BEFORE CRYOPRESERVATION AND AFTER THAWING FOR 3 DAYS, AND COOLED AT A COOLING RATE OF 25 K/MIN TO 203 K (-70 DEG C). THEN THEY WERE STORED IN LIQUID NITROGEN FOR 2 WEEKS. IN THE TEST OF GLUCOSE-STIMULATING INSULIN RELEASE AFTER THAWING, THE BASAL INSULIN RELEASE FROM CRYOPRESERVED ISLETS WAS ALMOST THE SAME AS THAT FROM THE CONTROL. WHEN CHALLENGED WITH HIGH GLUCOSE CONCENTRATION, THE INSULIN SECRETORY RATE OF THE CRYOPRESERVED ISLETS INCREASED ABOUT 3-FOLD. IT IS CONCLUDED THAT THE HAMSTER ISLETS CAN BE CRYOPRESERVED WITH GOOD FUNCTIONAL ABILITIES USING A FAIRLY RAPID COOLING RATE OF 25 K/MIN.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 1989-2524
  • Languages: Japanese
  • Source: Low Temperature Medicine - vol. 15 - n. 1
  • Publication date: 1989
  • Document available for consultation in the library of the IIR headquarters only.

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