Share

STUDIES ON CRYOPRESERVATION OF HAMSTER PANCREATIC ISLETS USING A FAIRLY RAPID COOLING RATE.

Author(s) : FUKUSHIMA W.

Type of article: Article

Summary

IN THE STUDY ON ISLET PRESERVATION INVOLVING A FREEZING METHOD, THE AUTHOR HAS INVESTIGATED THE POSSIBILITY OF CRYOPRESERVED ISLETS HAVING IMMUNOSUPPRESSIVE EFFECTS. ISOLATED HAM-STER ISLETS WERE COOLED AT THE RATE OF 25 K/MIN AND STORED IN LIQUID NITROGEN FOR 2 WEEKS BEFORE BEING THAWED IN A WATER BATH AT 310 K (37 DEG C). ISLETS WERE CULTURED FOR AT LEAST 3 DAYS BEFORE FREEZING AND 3 DAYS AFTER THAWING. ISOGENEIC AND XENOGENEIC TRANSPLANTATION OF CRYOPRESERVED AND CULTURED ISLETS WERE CARRIED OUT AND THE DURATION OF GRAFT SURVIVAL WAS OBSERVED. ISOGENEIC TRANSPLANTATION (HAMSTER TO HAMSTER) WAS SUCCESSFUL SINCE ALL DIABETIC HAMSTERS BECAME NORMOGLYCEMIC AND REMAINED SO UNTIL THE KIDNEYS BEARING ISLETS WERE REMOVED. XENOGENEIC TRANSPLANTATION (HAMSTER TO RAT) RESULTED IN POOR SUCCESS. IT IS CONCLUDED THAT CRYOPRESERVATION IS USEFUL IN ISOGENEIC ISLET TRANSPLANTATION, BUT THE COOLING RATE OF 25 K/MIN OFFERS NO IMMUNOLOGICAL ADVANTAGE IN XENOGENEIC TRANSPLANTATION.

Details

  • Original title: STUDIES ON CRYOPRESERVATION OF HAMSTER PANCREATIC ISLETS USING A FAIRLY RAPID COOLING RATE.
  • Record ID : 1991-0886
  • Languages: English
  • Source: Low Temperature Medicine - vol. 16 - n. 1
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (3)
See the source

Indexing