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Sugars exert a major influence on the vitrification properties of ethylene glycol-based solutions and have low toxicity to embryos and oocytes.

Author(s) : KULESHOVA L. L., MACFARLANE D. R., TROUNSON A. O., et al.

Type of article: Article

Summary

A systematic approach was used to assess the vitrification properties of ethylene glycol-based solutions supplemented with carbohydrates. Solutions were prepared using ethylene glycol as the cryoprotectant, 0.9% sodium chloride in water, and six different sugars: D-glucose, D(-)-fructose, D-sorbitol, sucrose, D(+)-trehalose, and raffinose. The authors conclude that added sugars do contribute to a solution's overall vitrification properties, and their properties should be taken into consideration when vitrification solutions are being designed or modified.

Details

  • Original title: Sugars exert a major influence on the vitrification properties of ethylene glycol-based solutions and have low toxicity to embryos and oocytes.
  • Record ID : 2000-1024
  • Languages: English
  • Source: Cryobiology - vol. 38 - n. 2
  • Publication date: 1999/03

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