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Survival of directionally solidified B-lymphoblasts under various crystal growth conditions.

Author(s) : HUBEL A., CRAVALHO E. G., NUNNER B., et al.

Type of article: Article

Summary

Reduction of temperature during freezing brings about two complex and interrelated phenomena: crystal nucleation and subsequent growth processes and change in biophysical properties of a biological system. The purpose of this investigation is to relate the morphology of the solid phase with the survival of a cell. Results indicated that, for the range of experimental conditions tested, a maximum survival of approximately 78% could be achieved using a temperature gradient of 25,000 K/min and an interface velocity of 23 micrometers/s (cooling rate: 35 K/min) for B-lymphoblasts. The results indicated that not only is the redistribution of solute during freezing a potential source of damage during freezing but ice-cell interactions are also. Thus, the cooling rate alone may not be adequate to describe the freezing process.

Details

  • Original title: Survival of directionally solidified B-lymphoblasts under various crystal growth conditions.
  • Record ID : 1993-0522
  • Languages: English
  • Source: Cryobiology - vol. 29 - n. 2
  • Publication date: 1992/04

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