The determination of membrane permeability coefficients of canine pancreatic islet cells and their application to islet cryopreservation.
Author(s) : LIU J., ZIEGER M. A. J., LAKEY J. R. T., WOODS E. J., CRITSER J. K.
Type of article: Article
Summary
Sufficient numbers of pancreatic islets for successful allotransplantation can be achieved by storing and then pooling islets from several donors. Optimal MHC matching and infectious disease screening also require long-term storage of islets. Cryopreservation protocols may be optimized by modeling. The objective of the present work was to determine experimentally the biophysical parameters of canine islet cells: the osmotically inactive cell volume, hydraulic conductivity, cryoprotectant permeability coefficient and the reflection coefficient. A determination of these parameters allows the simulation of cell responses using computer models.
Details
- Original title: The determination of membrane permeability coefficients of canine pancreatic islet cells and their application to islet cryopreservation.
- Record ID : 1998-3186
- Languages: English
- Source: Cryobiology - vol. 35 - n. 1
- Publication date: 1997/08
Links
See other articles in this issue (8)
See the source
Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cryobiology; Cell; Dog; Permeability; Pancreas; Modelling; Membrane; Cryopreservation
-
Osmotic characteristics of isolated human and c...
- Author(s) : WOODS E. J., ZIEGER M. A. J., LAKEY J. R. T., LIU J., CRITSER J. K.
- Date : 1997/09
- Languages : English
- Source: Cryobiology - vol. 35 - n. 2
View record
-
Effect of cryopreservation on the immunogenicit...
- Author(s) : IWASA K., IZUMI R., MIYAZAKI I.
- Date : 1996/01
- Languages : Japanese
- Source: Low Temperature Medicine - vol. 22 - n. 1
View record
-
TRANSPLANTATION OF CRYOPRESERVED AND FRESH RAT ...
- Author(s) : RAJOTTE R. V.
- Date : 1983
- Languages : English
- Source: Cryobiology - vol. 20 - n. 2
View record
-
AUTOTRANSPLANTATION OF PANCREATIC ISLETS ISOLAT...
- Author(s) : SEKINO H.
- Date : 1987
- Languages : Japanese
- Source: Low Temperature Medicine - vol. 13 - n. 2
View record
-
Current status and problems of pancreas preserv...
- Author(s) : MATSUMOTO S., KURODA Y., SAITOH Y.
- Date : 1996/09
- Languages : Japanese
- Source: Low Temperature Medicine - vol. 22 - n. 3
View record