Cryosurvival of goldfish embryo after subzero freezing.

Author(s) : LIU K.-C., CHOU T.-C., LIN H.-D.

Type of article: Article


Goldfish embryos of pre-morula, somite formation, optic vesicle, heart formation and pigment formation stages were tested for cryoprotectant toxicity, using DMSO and glycerol at 10, 12 and 15% concentration. Those that showed 50% or more survival were employed for cold shock tests, from 24 to 4 deg C at the rate of -1 deg C/min. Only embryos at the heart formation stage protected with 12% DMSO showed 60% survival and 35% hatching rates. A group of embryos of that stage went through a freezing and thawing test to temperatures of -15, -25 and -35 deg C. They showed 30, 5 and 2% survival rates and 12, 3 and 1% hatching rates, respectively.


  • Original title: Cryosurvival of goldfish embryo after subzero freezing.
  • Record ID : 1994-0580
  • Languages: English
  • Source: Aquat. living Resour./Ressour. vivantes aquat. - vol. 6 - n. 1
  • Publication date: 1993
  • Document available for consultation in the library of the IIR headquarters only.


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