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Assessment of gelatin hydrolysates from threadfin bream (Nemipterus hexodon) skin as a cryoprotectant for denaturation prevention of threadfin bream natural actomyosin subjected to different freeze-thaw cycles.

Author(s) : KUEPETHKAEW S., KLOMKLAO S., BENJAKUL S., BETTI M., SIMPSON B. K.

Type of article: IJR article

Summary

Gelatin hydrolysates prepared from threadfin bream skin (GH) using lizardfish stomach pepsin (GHLP) with different degrees of hydrolysis (DHs: 10–40%) were studied as a potential alternative to commercial cryoprotectant (sucrose/sorbitol blend; SuSo, 1:1) used in frozen surimi. The characteristics of threadfin bream natural actomyosin (NAM) containing 8% GHLP with different DHs or SuSo were compared to control NAM before and after freeze-thaw treatment. After 3 and 6 freeze-thaw cycles, all present cryoprotectants retarded the protein changes. Among all samples, the highest Ca2+-ATPase activity, total sulfhydryl and solubility with the coincidental lowest disulfide bond content were observed in NAM added with GHLP having 20% DH after repeated freeze-thawing. Also, GHLP could prevent lipid oxidation in NAM as shown by the lower TBARS value. When cryoprotective properties of GHLP with 20% DH and GH prepared by papain (GHP) (DHs: 10–40%) were compared, the cryoprotectant properties of GHLP were similar to that of GHP. However, GHP with 10% DH exhibited a higher protective effect on protein denaturation. Hence, both GHLP and GHP with suitable DH can be a new generation cryoprotectant with the lower sweetness in surimi products.

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Pages: 19-27

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Details

  • Original title: Assessment of gelatin hydrolysates from threadfin bream (Nemipterus hexodon) skin as a cryoprotectant for denaturation prevention of threadfin bream natural actomyosin subjected to different freeze-thaw cycles.
  • Record ID : 30030328
  • Languages: English
  • Subject: Technology
  • Source: International Journal of Refrigeration - Revue Internationale du Froid - vol. 143
  • Publication date: 2022/11
  • DOI: http://dx.doi.org/10.1016/j.ijrefrig.2022.06.027
  • Document available for consultation in the library of the IIR headquarters only.

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