Share

AUTOMATED COMPUTER ANALYSIS OF CELL SIZE CHANGES DURING CRYOMICROSCOPE FREEZING: A BIASED TRIDENT CONVOLUTION MASK TECHNIQUE.

Author(s) : DILLER K. R., KNOX J. M.

Type of article: Article

Summary

A SLOW SCAN PROCEDURE WAS USED FOR THE OFF LINE DIGITIZATION OF PHOTOMICROGRAPHS OF CELLS FROZEN ON A CRYOMICROSCOPE. IMAGES WERE PROCESSED TO IDENTIFY THE PERIMETER OF INDIVIDUAL CELLS IN ORDER TO ESTIMATE CHANGES IN VOLUME AS A FUNCTION OF TEMPERATURE DURING A FREEZE-THAW PROTOCOL. A NEW EDGE FINDING TECHNIQUE, USING A TRIDENT SHAPED MASK CONVOLVED WITH THE DIGITIZED IMAGE, WAS DEVELOPED TO TRACK CELL BOUNDARIES IN THE MIDST OF COMPLEX OVERLYING ICE STRUCTURES.

Details

  • Original title: AUTOMATED COMPUTER ANALYSIS OF CELL SIZE CHANGES DURING CRYOMICROSCOPE FREEZING: A BIASED TRIDENT CONVOLUTION MASK TECHNIQUE.
  • Record ID : 1983-2368
  • Languages: English
  • Source: Cryo-Letters - vol. 4 - n. 2
  • Publication date: 1983
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (5)
See the source

Indexing