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CATHARANTHUS CELL SUSPENSIONS OBSERVED DURING FREEZING AND THAWING WITH A CRYOMICROSCOPE.

Author(s) : GAZEAU C., JONDET M., DEREUDDRE J.

Type of article: Article

Summary

CELLS WERE PRECULTURED IN MANNITOL OR SORBITOL, TREATED WITH A CRYOPROTECTIVE AGENT, THEN SLOW COOLED (1 K/MIN) TO 233 K (-40 DEG C) AND REWARMED (5 K/MIN) TO 283 K (10 DEG C). DURING FREEZING, THE SHAPE AND THE GROWTH OF ICE CRYSTALS DEPENDED ON THE SEEDING TEMPERATURE OF THE EXTRACELLULAR MEDIUM. A RAPID FORMATION OF TINY CRYSTALS INSIDE UNPROTECTED CELLS WAS OBSERVED. THIS FLASHING LED TO THE DEATH OF CELLS. MECHANICAL EFFECTS INDUCED BY EXTRACELLULAR ICE CRYSTALS ALTER THE CELL MORPHOLOGY MORE OR LESS REVERSIBLY. DEHYDRATION OF CELLS WAS ACCOMPANIED BY SHRINKAGE AND VOLUME REDUCTION OF THE CRYOPROTECTED CELLS WITHOUT ANY INTRACELLULAR CRYSTALLIZATION. AMOEBA-LIKE DISTORTIONS OF PROTOPLASMA OCCURRED DURING FREEZING ; THEY WERE MORE EASILY OBSERVED DURING REWARMING: THESE DEFORMATIONS DISAPPEARED SLOWLY WITH CELL REHYDRATION. SOME CELLS SWELLED TO THEIR ORIGINAL VOLUMES ; OTHERS SWELLED TO A LARGER VOLUME THAN JUST BEFORE FREEZING.

Details

  • Original title: CATHARANTHUS CELL SUSPENSIONS OBSERVED DURING FREEZING AND THAWING WITH A CRYOMICROSCOPE.
  • Record ID : 1989-2520
  • Languages: English
  • Source: Cryo-Letters - vol. 10 - n. 2
  • Publication date: 1989/03
  • Document available for consultation in the library of the IIR headquarters only.

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