Share

CORRELATION BETWEEN INTRACELLULAR NUCLEATION TEMPERATURES AND CELL DAMAGE OF HUMAN LYMPHOCYTES IN THE PRESENCE OF VARIOUS CONCENTRATIONS OF DMSO DETERMINED BY CRYOMICROSCOPY.

Author(s) : ENGLICH S.

Type of article: Article

Summary

THE VIABILITY OF HUMAN LYMPHOCYTES FROZEN ON THE STAGE OF A CRYOMICROSCOPE WITH A CONSTANT RATE OF 100 K/MIN WAS MEASURED AS A FUNCTION OF THE LOWEST TEMPERATURES ATTAINED. THE EXPERIMENTS WERE PERFORMED AT DMSO CONCENTRATIONS OF 0, 5 AND 10% VOL/VOL. WITH INCREASING DMSO CONCENTRATIONS, BOTH INTRACELLULAR ICE FORMATION AND THE DROP IN CELL SURVIVAL ARE SHIFTED TO LOWER TEMPERATURES. THE TWITCHING TYPE OF INTRACELLULAR ICE FORMATION WAS OBSERVED IN THE RANGE OF HOMOGENEOUS NUCLEATION TEMPERATURES WHILE DARKENING WAS EXPLAINED IN TERMS OF HETEROGENEOUS NUCLEATION ACCORDING TO THE OBSERVED CRYSTALLIZATION TEMPERATURES.

Details

  • Original title: CORRELATION BETWEEN INTRACELLULAR NUCLEATION TEMPERATURES AND CELL DAMAGE OF HUMAN LYMPHOCYTES IN THE PRESENCE OF VARIOUS CONCENTRATIONS OF DMSO DETERMINED BY CRYOMICROSCOPY.
  • Record ID : 1986-2108
  • Languages: English
  • Source: Cryo-Letters - vol. 7 - n. 1
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (3)
See the source

Indexing