Share

BASIC INVESTIGATIONS ON THE FREEZING OF HUMAN LYMPHOCYTES.

Author(s) : SCHEIWE M. W., KORBER C.

Type of article: Article

Summary

HUMAN LYMPHOCYTES WERE FROZEN AT CONSTANT COOLING RATES IN THE RANGE 2.4 TO 1,000 K/MIN WITHOUT CRYOADDITIVE ON THE COLD STAGE OF A THERMALLY DEFINED CRYOMICROSCOPE. THE LIKELIHOOD OF THE FORMATION OF INTRACELLULAR ICE WAS DETERMINED AS A FUNCTION OF THE COOLING RATE. A THEORETICAL ANALYSIS OF THE CELL VOLUME LOSS DURING FREEZING WAS COMPARED TO THE EXPERIMENTAL DATA AND USED FOR AN INDIRECT DETERMINATION OF THE WATER PERMEABILITY OF THE CELLS. A RELATIVE OPTIMUM OF THE COOLING RATE IS PREDICTED THEORETICALLY UNDER THE ASSUMPTION OF A CRITICAL LEVEL OF INTRACELLULAR SALT CONCENTRATION NEAR THE EUTECTIC TEMPERATURE.

Details

  • Original title: BASIC INVESTIGATIONS ON THE FREEZING OF HUMAN LYMPHOCYTES.
  • Record ID : 1984-1245
  • Languages: English
  • Source: Cryobiology - vol. 20 - n. 3
  • Publication date: 1983

Links


See other articles in this issue (4)
See the source

Indexing