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CRYOMICROSCOPE INVESTIGATION AND THERMODYNAMIC MODELLING OF THE FREEZING OF UNFERTILIZED HAMSTER OVA.

Author(s) : SHABANA M., MCGRATH J. J.

Type of article: Article

Summary

THERMODYNAMIC COMPUTER MODELLING WAS USED TO PREDICT THE FREEZING RESPONSE OF SINGLE-CELLED UNFERTILIZED HAMSTE THE CELL MEMBRANE TRANSPORT CHARACTERISTICS WERE INVESTIGATED, USING A MICROSCOPE DIFFUSION CHAMBER SYSTEM. THE PREDICTED RESPONSE WAS COMPARED TO THE ACTUAL VOLUMETRIC RESPONSE OBSERVED DURING FREEZING ON A TEMPERATURE-CONTROLLED CRYOMICROSCOPE CONDUCTION STAGE. THE EFFECT OF THE COOLING RATE ON THE NUCLEATION TEMPERATURE OF UNPROTECTED OVA AND PROTECTED OVA SUSPENDED IN A 1.5 M DMSO SOLUTION WAS INVESTIGATED. OVERALL MEAN NUCLEATION TEMPERATURES OF 260 AND 215.9 K (-13 AND -57.1 DEG C) WERE OBSERVED FOR UNPROTECTED AND PROTECTED OVA, RESPECTIVELY, WHERE THE MEAN NUCLEATION TEMPERATURE FOR PROTECTED OVA WAS STRONGLY COOLING RATE DEPENDENT.

Details

  • Original title: CRYOMICROSCOPE INVESTIGATION AND THERMODYNAMIC MODELLING OF THE FREEZING OF UNFERTILIZED HAMSTER OVA.
  • Record ID : 1989-2080
  • Languages: English
  • Source: Cryobiology - vol. 25 - n. 4
  • Publication date: 1988/08

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