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MICROSCOPIC RESPONSE OF DOG PANCREAS BETA-CELLS TO FREEZING AND OSMOTIC STRESSES.

Author(s) : AGGARWAL S. J., DILLER K. R., DAWIDSON I. J.

Type of article: Article

Summary

THE FREEZE-THAW RESPONSE OF INDIVIDUAL DOG BETA-CELLS WAS INVESTIGATED ON A CRYOMICROSCOPE AT COOLING RATES OF 3, 10 AND 30 K/MIN TO 213 K (-60 DEG C) IN CONJUNCTION WITH A WARMING RATE OF 60 K/MIN. THE CELLS SHRANK TO APPROXIMATELY 60% OF THE INITIAL VOLUME AT THE SLOWEST COOLING RATE, TO A LESSER EXTENT AT THE INTERMEDIATE RATE, AND AT THE FASTEST RATE THERE WAS EVIDENCE OF INTRACELLULAR ICE FORMATION WITH VIRTUALLY NO CHANGE IN VOLUME. THE OSMOTIC RESPONSE OF THE CELLS WHEN EXPOSED TO ELEVATED SALT AND/OR GLYCEROL CONCENTRATIONS AT ROOM TEMPERATURE, INDICATED A SENSITIVITY TO DAMAGE IN SOLUTIONS ABOVE 750 MOSMOL. THE OSMOTICALLY INACTIVE VOLUME OF THE CELLS WAS DETERMINED TO BE 16% OF THE ISOTONIC VALUE.

Details

  • Original title: MICROSCOPIC RESPONSE OF DOG PANCREAS BETA-CELLS TO FREEZING AND OSMOTIC STRESSES.
  • Record ID : 1987-1609
  • Languages: English
  • Source: Cryo-Letters - vol. 7 - n. 4
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

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