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Cryopreservation of cell suspensions and embryogenic calluses of Citrus using a simplified freezing process.

Author(s) : ENGELMANN F., DAMBIER D., OLLITRAULT P.

Type of article: Article

Summary

A simplified cryopreservation process was experimented with a cell suspension of willow leaf mandarin and embryogenic calluses of six varieties of Citrus. Its efficiency was comparable to that of the standard freezing protocol developed previously for these materials, which required the use of a programmable freezing apparatus. The cell suspension could be frozen without modifying the original pretreatment conditions (0.15 M sucrose + 5% DMSO). Embryogenic calluses of five varieties out of the six experimented withstood cryopreservation with the simplified freezing process. Optimal results were obtained by increasing the DMSO concentration to 10 or 15%.

Details

  • Original title: Cryopreservation of cell suspensions and embryogenic calluses of Citrus using a simplified freezing process.
  • Record ID : 1994-2306
  • Languages: English
  • Source: Cryo-Letters - vol. 15 - n. 1
  • Publication date: 1994/01
  • Document available for consultation in the library of the IIR headquarters only.

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