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Advantages of cryopreservation of cell suspensions and embryogenic calli for citrus breeding programmes.

Author(s) : ENGELMANN F., AGUILAR M. E., DAMBIER D., CABASSON C., MICHAUX-FERRIERE N., OLLITRAULT P.

Type of article: Article

Summary

The best results were obtained by pretreatment with sucrose and DMSO followed by prefreezing to -40 deg C. In vitro plantlets morphologically similar to unfrozen controls were produced from cryopreserved suspensions. It was found that a 10-month storage period at -196 deg C did not modify cell survival.

Details

  • Original title: Advantages of cryopreservation of cell suspensions and embryogenic calli for citrus breeding programmes.
  • Record ID : 1995-1629
  • Languages: English
  • Source: Fruits - vol. 49 - n. 1
  • Publication date: 1994/01
  • Document available for consultation in the library of the IIR headquarters only.

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