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CRYOPRESERVATION OF HUMAN PLATELETS WITH GLYCEROL-GLUCOSE SUSPENDED IN A LARGE FLUID VOLUME: INFLUENCE OF THE COOLING RATE.

Author(s) : SCHEIWE M. W.

Type of article: Article

Summary

PLATELETS HAVE BEEN PREPARED FOR FREEZING BY SLIGHT VARIATION OF THE METHOD ORIGINALLY DESCRIBED BY DAYIAN AND ROWE. RECOVERY AND IN VITRO TESTING WERE DETERMINED. SAMPLES CONTAINING 100 ML WERE FROZEN IN PLASTIC BAGS WITHIN A METAL CONTAINER, PROVIDING 5 MM THICKNESS OF THE LIQUID LAYER. WELL-DEFINED LINEAR COOLING RATES INSIDE THE SAMPLES IN THE WHOLE RANGE FROM 7 TO 70K/MIN WERE GENERATED BY MEANS OF A SPECIAL FREEZER; THE RESULTS INDICATE OPTIMAL COOLING RATES NEAR 30 TO 40K/MIN. IT IS CONCLUDED THAT SIMILAR TO OTHER BLOOD CELLS THE FREEZING INJURY OF PLATELETS CAN BE EXPLAINED BY THE TWO-FACTOR HYPOTHESIS.

Details

  • Original title: CRYOPRESERVATION OF HUMAN PLATELETS WITH GLYCEROL-GLUCOSE SUSPENDED IN A LARGE FLUID VOLUME: INFLUENCE OF THE COOLING RATE.
  • Record ID : 1982-1046
  • Languages: English
  • Source: Cryo-Letters - vol. 2 - n. 8
  • Publication date: 1981
  • Document available for consultation in the library of the IIR headquarters only.

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