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THE EFFECT OF COOLING RATE AND WARMING RATE ON THE PACKING EFFECT IN HUMAN ERYTHROCYTES FROZEN AND THAWED IN THE PRESENCE OF 2 M GLYCEROL.

Author(s) : PEGG D. E.

Type of article: Article

Summary

THE EFFECT OF HEMATOCRIT (2 VERSUS 75%) HAS BEEN STUDIED ON HUMAN RED BLOOD CELLS FROZEN AND THAWED IN 2 M GLYCEROL AT A RANGE OF COOLING RATES (0.8-850 K/MIN) AND WARMING RATES (0.1-200 K/MIN). THE RESULTS AT A HEMATOCRIT OF 75% SHOW A DECREASE IN RECOVERY WITH INCREASED CELL PACKING, PRIMARILY DEPENDENT ON WARMING RATE AT COOLING RATES LOWER THAN 100 K/MIN AND ON COOLING RATE AT HIGHER COOLING RATES. RAPID WARMING REDUCED THE PACKING EFFECT, WHEREAS COOLING FASTER THAN 100 K/MIN ACCENTUATED IT. IT HAS BEEN SUGGESTED THAT THEY MAY BE EXPLAINED BY EFFECTS OF COOLING AND WARMING RATES ON THE DIMENSIONS OF THE LIQUID CHANNELS IN WHICH THE CELLS ARE ACCOMMODATED DURING FREEZING AND THAWING.

Details

  • Original title: THE EFFECT OF COOLING RATE AND WARMING RATE ON THE PACKING EFFECT IN HUMAN ERYTHROCYTES FROZEN AND THAWED IN THE PRESENCE OF 2 M GLYCEROL.
  • Record ID : 1985-1299
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 5
  • Publication date: 1984

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