Share

SOLUTION AND INTRACELLULAR ICE EFFECT INJURY IN FREEZE-PRESERVATION OF GRANULOCYTES.

Author(s) : SCHEIWE M. W., KORBER C., ENGLICH S.

Type of article: Article

Summary

HUMAN GRANULOCYTES IN SALINE WERE FROZEN AT RATES BETWEEN 5 AND 100 K/MIN AND THAWED AT 100 K/MIN THE FINAL COOLING TEMPERATURE BEING VARIED. A SIMULTANEOUSLY APPLIED FLUORESCENCE VIABILITY ASSAY SERVED AS THE CRITERION TO DETERMINE THE PERCENTAGE OF LIVING AND DEAD CELLS. MAXIMUM SURVIVAL WAS FOUND AT THE RATE OF 20 K/MIN WHEN COOLING TO 213 K (-60 DEG C) OR LOWER. THE TEMPERATURE OF 239 K (-34 DEG C) IS THE ONSET OF DAMAGE BY INTRACELLULAR ICE FORMATION. IT IS CONCLUDED THAT INTRACELLULAR ICE FORMATION IS LETHAL, BUT NOT DARKENING OF GRANULOCYTES.

Details

  • Original title: SOLUTION AND INTRACELLULAR ICE EFFECT INJURY IN FREEZE-PRESERVATION OF GRANULOCYTES.
  • Record ID : 1985-0822
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 3
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (4)
See the source

Indexing