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INTRACELLULAR FREEZING OF HUMAN GRANULOCYTES.

Author(s) : SCHWARTZ G. J., DILLER K. R.

Type of article: Article

Summary

HUMAN GRANULOCYTE SUSPENSIONS WERE EXPOSED TO CONTROLLED FREEZING REGIMENS ON A CRYOMICROSCOPE, AND THE INCIDENCE OF INTRACELLULAR FREEZING WAS MEASURED AS A FUNCTION OF COOLING RATE AND EXTRACELLULAR NUCLEATION TEMPERATURE. THE PRESENCE OF INTRACELLULAR ICE WAS ASSESSED BY ANALYSIS OF SERIALLY RECORDED IMAGES OF THE FREEZE-THAW PROCESS AND BY CORRELATION WITH MEASURED PATTERNS OF CHANGE IN THE CELL VOLUME. A THRESHOLD WAS DESCRIBED FOR INTRACELLULAR FREEZING AS AN EMPIRICAL FUNCTION OF COOLING RATE AND EXTRACELLULAR NUCLEATION TEMPERATURE.

Details

  • Original title: INTRACELLULAR FREEZING OF HUMAN GRANULOCYTES.
  • Record ID : 1985-2230
  • Languages: English
  • Source: Cryobiology - vol. 21 - n. 6
  • Publication date: 1984

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