ShareINTRACELLULAR FREEZING OF HUMAN GRANULOCYTES.
Author(s) : SCHWARTZ G. J., DILLER K. R.
Type of article: Article
Summary
HUMAN GRANULOCYTE SUSPENSIONS WERE EXPOSED TO CONTROLLED FREEZING REGIMENS ON A CRYOMICROSCOPE, AND THE INCIDENCE OF INTRACELLULAR FREEZING WAS MEASURED AS A FUNCTION OF COOLING RATE AND EXTRACELLULAR NUCLEATION TEMPERATURE. THE PRESENCE OF INTRACELLULAR ICE WAS ASSESSED BY ANALYSIS OF SERIALLY RECORDED IMAGES OF THE FREEZE-THAW PROCESS AND BY CORRELATION WITH MEASURED PATTERNS OF CHANGE IN THE CELL VOLUME. A THRESHOLD WAS DESCRIBED FOR INTRACELLULAR FREEZING AS AN EMPIRICAL FUNCTION OF COOLING RATE AND EXTRACELLULAR NUCLEATION TEMPERATURE.
Details
- Original title: INTRACELLULAR FREEZING OF HUMAN GRANULOCYTES.
- Record ID : 1985-2230
- Languages: English
- Source: Cryobiology - vol. 21 - n. 6
- Publication date: 1984
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Ice; Freezing rate; Survival; Blood; Granulocyte; Cryopreservation
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- Date : 1984
- Languages : English
- Source: Cryo-Letters - vol. 5 - n. 3
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- Author(s) : SCHEIWE M. W., KORBER C., ENGLICH S.
- Date : 1984
- Languages : English
- Source: Cryo-Letters - vol. 5 - n. 5
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- Author(s) : SPUTTEK A.
- Date : 1987
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- Source: Cryo-Letters - vol. 8 - n. 4
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- Author(s) : MEHL P., BOUTRON P.
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THE EFFECT OF COOLING RATE AND WARMING RATE ON ...
- Author(s) : PEGG D. E.
- Date : 1984
- Languages : English
- Source: Cryobiology - vol. 21 - n. 5
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