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Cryopreservation of in vitro sugar beet shoot-tips using the encapsulation-dehydration technique: development of a basic protocol.

Author(s) : VANDENBUSSCHE B., PROFT M. P. de

Type of article: Article

Summary

Shoot-tips were dissected from in vitro plantlets of sugar beet (Beta vulgaris L.) and placed for 24 hours on a sterile filter paper soaked with liquid medium. After encapsulation, the beads were precultured in liquid medium enriched with sucrose (stepwise increase up to 1.0 M). The beads were subsequently dehydrated using silicagel, placed in cryotubes and directly plunged into liquid nitrogen. A survival rate after freezing of 29% was obtained when a water content of 20-21% was reached. A similar survival rate of 20% was obtained for another clone.

Details

  • Original title: Cryopreservation of in vitro sugar beet shoot-tips using the encapsulation-dehydration technique: development of a basic protocol.
  • Record ID : 1997-0838
  • Languages: English
  • Source: Cryo-Letters - vol. 17 - n. 3
  • Publication date: 1996/05
  • Document available for consultation in the library of the IIR headquarters only.

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