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Enhanced formation of roots and subsequent promotion of growth of shoots on cryopreserved nodal segments of Asparagus officinalis L.

Author(s) : SUZUKI T., KANEKO M., HARADA T., YAKUWA T.

Type of article: Article

Summary

The increased frequency of root formation from cryopreserved nodal segments appears to have been induced by the freeze-thaw step of the cryopreservation procedure. The promotion of growth of shoots from cryopreserved nodal segments seemed to have been due to the increased percentage of root formation. Histological observations revealed that only dome-shaped meristematic tissue and a few cells of cladophyll primordia survived in cryopreserved nodal segments that had been cultured for 5 days after thawing. Disorganization and/or physiological changes might have occured in the surviving tissues and/or cells of the cryopreserved nodal segments that were responsible for the subsequent increased formation of roots.

Details

  • Original title: Enhanced formation of roots and subsequent promotion of growth of shoots on cryopreserved nodal segments of Asparagus officinalis L.
  • Record ID : 1999-3246
  • Languages: English
  • Source: Cryobiology - vol. 36 - n. 3
  • Publication date: 1998/05

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